【摘要】 本文报道一种新的求K_D的实验方法(简称微分法)。本法公式是根据药物—受体相互作用的占领学说,用数学方法推导而的。只需用药物的两个不同浓度,做两根单收缩曲线。在同一大白鼠左侧离体输精管标本上,用该法先后求得重酒石酸去甲肾上腺素的两个K_D值,也用累积剂量一效应曲线法简称累积法求得两个;共17个标本,各求得17对K_D值。两方法先后四次实验所求得的K_D均值之间,除累积法第二次的均值分别与微分法先后两次均值相比,差别显著外,其余均不显著。经方差齐性检验,本法17对K_D值的变异,显著小于累积法。本法不仅操作、计算简便,重现性好,精密度高,而且求得的K_D值也与累积法相符。更多还原

【Abstract】 In this paper a new experimental method of evaluation of K_D is reported. The equation is derived from the occupation theory of drug-recesptor interaction using mathematical method. Through the experiments of rat’s deferent duct specimens in vitro, treated with noradrenaline bitartrate, it is demonstrated that this new method is superior to the method of cummulative dose-response curve in simplicity, reprodu-cibility, and accuracy. In addition the K_D thus obtained is also in accordance with that of the latter.更多还原