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应用固相抗HBs双抗体ELISA技术检测慢性HB患者血清中HBsAg特异性免疫复合物

The Detection of HBsAg--specific CIC in Patients with Chronic HB by Double Antibody ELISA Using Anti--HBs Attached on Solid Phase

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【摘要】 介绍一种应用固相抗HBs-BLISA技术分类检测IgG和IgM类抗体形成的HBsAg特异性CIC的方法。本方法最适检出抗原稍过量的HBsAg-CIC。对不同类型慢性HB患者检测的结果表明,HBAC、CAH较CPH的HBsAg-CIC检出率高,且IgM类抗体形成的HBsAg—CIC与病型区别和活动性肝损害的关系更为密切。

【Abstract】 An assay for the specific detection of HBsAg-CIC composed of different immunoglobulins (IgG and IgM) by ELISA using anti-HBs attached on solid phase was reported in this paper. thismethod was most suitable for the detection of HBsAg-CIC with slightly exce- ssive antigen. The results of the detection in different types of patients with chronic HB showed that the incidence of HBsAg-CIC in HBAC and CAH was remarkably higher than that in CPH, in ad- dition, BHsAg/IgM was more close- ly related with the difference of disease types and with the activity of liver damage.

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