【摘要】 以扫描和冰冻蚀刻法电镜技术观察冷冻前后的家兔精子形态变化。兔精子冷冻后,顶体赤道段及其附近首先出现肿胀,其头部质膜、顶体膜特别易于破裂和部分脱落,而顶体肿胀向前方突出的现象不常见。据冰冻蚀刻复型膜观察所见,正常兔精子头部质膜PF面,依其颗粒多少可划分为较光滑的顶脊区;颗粒最多,呈疏密相间散在分布的中央扁平区和颗粒稀少的核后帽区;并在头基部,由较粗大颗粒组成的索状物呈平行或斜行排列环绕周围。不同平面劈裂的核内染色质大多为有规则平行排列的索状结构,仅近基结部者有杂乱排列的现象。经冷冻后,顶脊区质膜PF面颗粒增加,中央区首先出现块状隆起,继而核后帽前区出现小长方型隆起,颗粒增加。头基部索状物颗粒有分散现象,并向前部迁移。此外,兔精子的颈部及相邻的中段常出现局部严重肿胀和质膜破损。更多还原

【Abstract】 The ultrastructure of rabbit sperm and its changes after freezing were observed and described under scanning electron microscopy. After freezing, the equatorial segment and its vicinity of rabbit sperm became swollen. The plasma membrane and external membrane of acrosome were liably to rupture and detached partly from the head The swelled acrosome protruded forwards occurred rarely. According to the results of freeze-etching, as shown in pictures, the pF-face of plasma membrane could be divided into three regions: the relatively smooth region of acrosome ridge; the flat centreral region of acrosome particularly rich in particles with uneven density scattered randomly and the post aerosomal cap region with less scattered particles Surrounding the basal part of sperm head there were numerous striate cords consisting of course particles in parallel or oblique arrange ment. Most of chromatine in the nucleus of different etching-surfaces was a parallely arranged cord shaped structure, but near the head basal area the cord shaped structure became irregular. After freezing, the particles increase on the plasma membrane of acrosomal ridge region and in the anterior region of post nucleal cap there occurred the swelled rectangle bulges and the particles increase as well. The cord shaped structure scattered and the particles seemingly migrated towords the post nucleal region. In addition, some parts of the sperm neck and middle piece frequently swelled and the plasma membrane was impaired or ruptured.更多还原