【摘要】 应用间接血凝试验、微量补体结合试验、对流免疫电泳、琼脂双向扩散试验和团集反应,对镜检有虫的25份阳性血清、非疫区22份阴性血清及疫区1070份待检血清进行伊氏锥虫病血清学诊断比较研究。 实验结果表明:间接血凝试验特异性强,敏感性高,是较理想的血清学诊断方法。其次是微量补体结合试验和琼脂双向扩散。对流免疫电泳和团集反应不但有假阴性,而且有假阳性,诊断价值相对较差。更多还原

【Abstract】 A comparative study of some serodiagnostic assays for "Surra" was undertaken. The indirect haemagglutination test (IHAT), agar double diffusion test (ADDT), counterimmuno-electrophoresis test(CIET), microcomplement fixation test (MCFT), and agglutination test (AT) were compared. Serum samples from 25 buffalo with natural T. evansi infections confirmed by blood smears, and 1070 serum samples from buffalo in an epidemic area were tested, with 22 bovine serum samples from non-epidemic area as negative controls.The results showed that the IHAT was thebest sercdiagnostic technique for detecting T. evansi infection in the water buffalo because of its high specificity and sensitivity. The results from MCFT and ADDT were sometimes satisfactory. The CIET and CT gave both false positive and false negative results and were not the techniques of choice.更多还原