【摘要】 以苯酚:乙醇:碱酸缓冲液(30g:10ml:10ml)作展开剂,用单相下行纸层折法成功地分离了小白鼠脑中γ—氨基丁酸(GABA)、天冬氨酸(Asp),谷氨酸(Glu)和谷氨酰胺(Gln),并测定了其含量,分别为2.15±0.13、3.89±0.18、10.91±0.71、4.92±0.39umol/g wet wt。上述氨基酸的回收率均在90％以上。方法简便、稳定可靠、对比性好,与同类方法相比省时、灵敏度较高。更多还原

【Abstract】 The transmitter amino acids,γ-aminobutyric acid, aspartate,glutamate, and glutamine in mouse brain were successfully isolated by means of unid mensi- onal descending paper chromatography with a solvent composed of phenol, alcohol:borate buffer (30g: 10ml:10ml), and quantitatively analyzed, the contents of them being 2.15±0.13,3.89±0.18, 10.91±0.71 and 4.92±0.39 (μmol/g wet wt) respectively. The rates of recovery of these amino acids were all over 90%. The method was simple, stableand reliable, with good contrast and, compared with other similar methods, timesaving and more sensitive.更多还原