【摘要】 作者以U₁₄（小鼠宫颈癌第14号）细胞为抗原,免疫BALB/C小鼠,取其脾细胞与NS—1小鼠骨髓瘤细胞在PEG（聚乙二醇）作用下进行细胞融合。所形成的淋巴细胞杂交瘤细胞能生长于一种选择性培养液—HAT培养液中。对于具有分泌特异抗体的杂交瘤细胞,借助其上清液的免疫荧光试验加以选择。将间接膜免疫荧光试验阳性的杂交瘤细胞用有限稀释法进行克隆和扩大培养,得到1株专一分泌抗U₁₄单克隆抗体的淋巴细胞杂交瘤,定名为AU_14-1。杂交瘤细胞正继续在培养中。更多还原

【Abstract】 BALB/C mice were immunized with U₁₄ （mouse uterine cervical carcin- oma NO.14） cells as antigens and their spleen cells were hybridized with NS-1 mouse myeloma cells using PEG （polyethylene glycol） as the fusing agent. Hybrid cells grew in HAT medium. Hybridomas secreting specific antibodies were selected by means of immunofluorescence test of their super- natants. Hybridomas that were classified as positive by using indirect mem- brane immunofluorescence test were cloned by limting dilution and expand- ed by culture. A hybridoma, AU_14-1, secreted specifically antibody to an antigen present in uterine cervical carcinomas.Hybridoma is mainta-ined continuously in culture.更多还原