【摘要】 本文报告HSF 79-2二倍体成纤维细胞系,于体外保持较长时期生长增殖的特性,以及其形成组织样结构的初步结果。 HSF79-2细胞系来自17岁女性卵巢癌患者的腹部正常皮肤。原代采用组织块培养法,培养液系McCoy 5 A补加10～25％小牛血清。细胞系以1:2分种率传代、46～54代终止。此系细胞可停止传代,保持原瓶换液而长期生长(观察16个月),并增殖成厚膜。经组织学检查为结缔组织样结构。非传代瓶中保留细胞分别于3,6,12个月后重新传代,细胞的生长特性与二倍体性未变。其总的生存代数与连续传代细胞大致相等。上述特点,可能为二倍体成纤维细胞所共有,可用于保存某些细胞系,为研究细胞的运动、分化提供模型。更多还原

【Abstract】 A human diploid fibroblast cell line derived from normal abdominal skin of a 17-year-old female patient with ovary carcinoma was established and designated as HSF 79-2. The cultivation of skin fragments was employed in the primary explantation. The medium used was McCoy 5A supplemented with 15～25% calf serum. The grown fibroblasts were succesively subcultured at 1 to 2 split and stopped at the 45th to 54th passage.Having reached confluence the cells still had the ability to divide during 16 months observation if the medium was changed periodically. They displayed the property of dense growth and forming heavy, multilayer sheets, which became a visible membrane to the naked eyes. Under histological examination the membrane had the appearance of connective tissue. Part of the cells were subcultured again after 3,6, and 12 months maintenance in the same culture flask. Their growth character, ploidy, and the generation time were similar to that of the cells passaged normally.The characteristics of this cell line mentioned above appear to be shared by other diploid fibroblasts. It might be used for preserving cell lines and as a model for studying cell motility and differentiation.更多还原