【摘要】 本研究以羟基磷灰石(Hydroxyla patite)为吸附剂的柱层析法分离和纯化了中国产紫菜属(Porphyra)6种紫菜——条斑紫菜(P.yezoensis)、少精紫菜(P.oligospermatangia)、华北半叶紫菜(P.katadai var.hemiphylla)、甘紫菜(P.tenera)、坛紫菜(P.haitanensis)和边紫菜(P.marginata)——中的藻胆蛋白。吸收光谱测定的结果显示,R-藻红蛋白(R-phycoerythrin)和变藻蓝蛋白(Allophycocyanin)存在于所研究的6种紫菜中,而C-藻蓝蛋白(C-phycocyanin)只出现于甘紫菜、条斑紫菜、少精紫菜和华北半叶紫菜中,而R-藻蓝蛋白(R-phycocyanin)则只出现于坛紫菜和边紫菜中。更多还原

【Abstract】 Phycobiliproteins were separated and purified from six species of Porphyra, P. yesoensis, P. oligospermatangia, P. tenera, P. katadai var. hemiphylla, P. haitanensis and P. marginata, by the hydroxylapatite column chromatoigraphy. Their absorption Spectra were measured with Shimadzu UV-240 spectrophotometer.It was found that B-phycoerythrin and allophycocyanin are present in all the species investigated, whereas C-phycocyanin is present only in P. tenera, P. yezoensis, P. oligospermatangia and P. katadai var. hemiphylla, and K-phycocyanin only in P. mar-ginata and P. haitanensis.更多还原