【摘要】 <正> 电镜的高分辨和高放大性能,能够精确反映被观察客体真实结构。传统电子显微术强调,活体标本由离开母组织至固定的间隔时间应在几十秒之内,一般不能超过1分钟。这种取材原则,是试图避免死后自溶造成人工假象,从而达到保持组织细胞各种成份的天然结构的目的。但在生理学上,任何生活细胞对取材的机械等一些刺激都会发生不同程度的应激反应。这在具有收缩功能的心肌、骨骼肌和平滑肌等组织表现得最为显著,以致常常难以与病理性反应相鉴别,甚至造成诊断错误。本文就更多还原

【Abstract】 The experiment shows that abnormal contractile band usually occurs whenthe living specimens are taken from the myocardium of patients with myocar-dial diseases,animals and the intestins with different methods.This bandresults in the damage and disarrangment of cellular components,which lea-ds to the failure in research.It is a kind of irritable response caused by thestimulation during sampling and involving in the area about 0.5～0.6mm fromthe edge to the center of samples.The article presents that this kindof the artificial damage may be excluded.更多还原