【摘要】 经眶后静脉窦途径移植小鼠骨髓细胞以检测多能造血干细胞(CFU-S)。应用此法检测CFU-S时,注入的骨髓有核细胞数与生成的脾结节数之间是良好的线性关系。与经典的尾静脉注入途径相比较,其优点是:容易掌握方法,操作时间缩短,成功率高,特别是用黑色或棕色小鼠作注射时,更能体现眶后静脉窦注射的这一优点。更多还原

【Abstract】 According to the anatomic characteristics of the mouse retrorbital region a method of injecting the bone marrow cell suspension into blood stream through the retrorbital sinus (ROS) was established for CFU-S assay. To test the efficiency of the ROS injection, B6D2F1 bone marrow cell suspensions 0.25 ml with different cell concentration were injected into 86 irradiated (1,000 rads 60Co) B6D2F1 female mice via ROS. The same experiment was done with LACA female mice. The results showed that there was a linear correlation between the number of cells injected and colonies formed per spleen. A parallel comparison of ROS injection and tail vein injection of different bone marrow cell concentration was done in 120 LACA mice. In both groups the spleen colony number increased linearly with increasing cell concentration. The advantages of the ROS injection as compared with tail vein injection are discussed.更多还原