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实验性兔脑水肿脑组织的组织化学研究——Ⅰ.大脑脱氢酶的动态变化

HISTOCHEMICAL STUDY OF RABBIT ACUTE BRAIN EDEMAⅠ. DYNAMIC CHANGES OF CEREBRAL DEHYDROGENASES

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【作者】 韩英士; 刘齐良; 罗正兰; 胡全美; 虞佩兰; 吴中匡; 沈菊英; 何仲春;

【Author】 Han Yingshi Liu Qiliang Luo ZhenglanDepartment of Histology and Embryology, Hunan Medical CollegeHu Quanmei Yu Peilan Wu Zhongkuang Shen JuyingDepartment of Pediatrics, The First Affiliated Hospital, Hunan Medical CollegeHe ZhongchunInstitute of Zhuzhou Cement Carbide Works

【机构】 湖南医学院组织学与胚胎学教研室; 湖南医学院第一附属医院儿科; 株洲硬质合金厂研究所;

【摘要】 对伤寒内毒素所致兔脑水肿大脑皮髓质、脉络丛分泌上皮及小血管壁的SDH、ICDH、LDH及MAO 活性的变化进行了动态观察,并用图象分析仪对大脑皮质内SAH及LDH活性进行了相对定量测量。发现四种脱氢酶活性均有改变,且皮质内出现多个局灶性酶活性减弱区。

【Abstract】 The histochemical changes of dehydrogenases including SDH, LDH, ICDH and MAO were studied in the cortex and medulla, the secretory epithelial cells of choroid plexus and the wall of small blood vessels of the rabbit cerebrum with experimental acute brain edema induced by typhoid endotoxin. The relative quantitative analysis of SDH and LDH activity in the cerebral cortex were determined by Image Analyzer QTM-720. Histochemical changes of these four dehydrogenases and focal regions with diminished enzyme activity occurred in the cortex.

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