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长沙株洲两地秋冬季婴幼儿急性胃肠炎的病原学研究 Ⅰ、以酶联免疫吸附试验检测轮状病毒感染

STUDIES ON THE ETIOLOGY OF INFANTILE ACUTE GASTROENTERITIS IN CHANGSHA AND ZHUZHOU Ⅰ. ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTINC ROTA VIRUS INFECTION

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【作者】 李镇辉邬若楠胡光帜张天庭

【Author】 Li Zhenhui Wu Ruonan Hu GuangzhiThe Central Research Laboratory, The First Affiliated Hospital Hunan Medical collegeZhang Tianting Department of pediatrics, The First Affiliated Hospital,Hunan Medical College

【机构】 湖南医学院第一附属医院中心实验研究室湖南医学院第一附属医院儿科

【摘要】 用酶联免疫吸附试验对长沙、株洲两地82年及83年秋冬季婴幼儿急性胃肠炎住院患儿 95例的粪便进行轮状病毒抗原检测,阳性率为74.7%;其中82年50例标本同时作了电镜法的比较。此外对某妇幼保健院产科婴儿室发生的新生儿流行性腹泻30例及产房工作人员18人的粪便作了轮状病毒的酶联法检测,结果均为阴性。

【Abstract】 Enzyme-linked immunosorbent assay(ELISA)was used to detect the fecal antigen of rotavirus in the specimens of 95 infants and young children who had suffered from acute enteritis and admitted to the hospitals in Changsha and Zhuzhou. 71 specimens(74,7%)showed a positive result.A comparison was made between the ELISA technique and electron microscopy of the fecal specimens. Fecal specimens of 50 cases in 1982 were examined and 80%was positive by the former technique and 84% by the latter.In addition,in an epidemic of acute diarrhea affecting 20 neonates in the Maternity Hospital of Changsha, rotavirus was detected neither in the neonated nor in the infant-care personnel.

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