【摘要】 以鸭胚成纤维细胞——水泡性口膜炎病毒作为鸭干扰素的检测系统,证明北京鸭的脾脏细胞和全血细胞在诱生剂作用下,能够产生鸭外源性干扰素。考虑干扰素的抗病毒活性的效价、操作的简便和提纯的效果,以脾脏细胞诱生法优于全血细胞诱生法。以正交表L9(3~4)安排试验,表明诱生鸭干扰素的最佳条件组合是:水解乳蛋白液——2×10~7脾细胞/毫升——NDV—F系,39℃旋转培养20～24小时,鸭脾干扰素的滴度可达2,000单位/毫升以上.10％的小牛血清对于诱生鸭干扰素是必需的。理化和生物学特性的测定证明本研究的抗病毒物质是鸭干扰素。硫酸铵分级盐析法可以对鸭干扰素进行浓缩和粗提纯,滴度可以提高到10,000单位/毫升。更多还原

【Abstract】 The present study demonstrated that , using the duck embryo fibroblast——vesicularstomatitis virus system for assay of duck interferon, duck spleen cells and whole blood cells from healthy Beijing ducks could be induced to produce exogenous interferon and to be used as cell sources for their production . Taking into account the tiue of the antiviral activity, simplicity of manipulation and efficiency of purification , the method of inducing duck interferferon with spleen cells was found to be superior to that using duck whole blood cells.The L,(34)orthogonal comparison tests(three kinds of cell culture media, Lactalbumin hydrolysate, M199 , RPMI 1640; three spleen leukocyte concentrations, 2 ×107/ml, 1 ×107/ml, 0.5×107/ml; three inducers of interferon , NDV strain F, Sendai virus, Poly I : C) indicated that the best combination of the conditions for inducingduck spleen interferon was Lactalbumin hydrolysate —— 2 ×107/ml —— NDV strain F .Ten per cent new-born calf serum in the culture media was essential to induce duck interferon. Using this combination, after 20-24 hours’incubation at 39℃ on a roller drum , the titre of duck spleen interferon could consistently reach above 2000u/ml. Assay of the physio-chemical properies and biological activities indicated that the antiviral substances of the present study could be identified as the duck interferon a.The method of salting out with ammonium sulfate could concentrate and purify the duck interferon, and the titre of the final concentrated product could reach 10000u/ml.更多还原