【摘要】 琼脂对流免疫电泳是一种检测HBsAg常用方法,但存在的问题是其敏感度较低。笔者在常规方法的基础上,做了些改进:用琼脂糖代替纯化琼脂;并先将诊断血清加在阳极端孔内,预电泳15—20分钟,然后将待检标本加入阴极端孔内继续电泳50分钟。应用这种预电泳法检查416份标本并和常规法作了比较。结果表明预电泳法HBsAg检出率(25.0％)明显地高于常规法(15.4％)。更多还原

【Abstract】 Counter-immuuo-electrophoresis (CEP) is one of the most common methodsused to identify HBsAg, but it’s lower sensitivity has still been a problem.The c oaventioaal method was improved by using agarose as supporting mediuminstead pured agar and adopting pre-electrophoresis in order to make it moresensitive. The method of pre-electrophoresis was made as follows:Diagnostic serum was added to the wells at the positive end of argarose plate first and was electrophoresised for 15 to 20min.Then the samples of sera were added to the wells at the negative end of agarose plate at once and continued to run for 50 min.By using pre-CEP, 416 samples were examined.The results showed that pre-CEP had a higher HBSAg pasitive rate (25.0%) than the conventional method did (15.4% ).更多还原