【摘要】 <正> 固氮酶是由Mo-Fe蛋白和Fe蛋白组成的。在Mo-Fe蛋白中含有二个Mo原子。在固氮酶的固氮反应机制中,Mo原子是络合底物、催化底物还原的活性中心原子。故研究Mo在Mo-Fe蛋白中的可能结构显得十分重要。更多还原

【Abstract】 N2ase is made up of two distinct and separately isolated proteins. An Fe protein and an Mo-Fe protein that has two Mo atoms per mole. 99Mo decays to excited nuclear state of 99Tc. The angular correlation of the 741-181keV γ rays were emitted subsequently in the deexcitation of the 99Tc through the medial state 5/2 + . 99Mo was incorporated in N2ase by jncubation. After freezing the solution of 99Mo labelled N2ase the attenuation factor G2 of PAC and quadrapole frequency ωe were determind. Compared with the G2 and ωe of other 99Mo labelled complexes, the oxidation state and coordination structure of 99Mo in N2ase were speculated. The mechanism of nitrogen fixation by N2ase was also discussed according to the principle of electron transformation.更多还原