【摘要】 用4～20％管状聚丙烯酰胺梯度凝胶电泳,分离肝癌病人血清5′-核苷酸磷酸二酯酶同工酶,出现的11条同工酶带中,第Ⅰ、Ⅴ和第Ⅵ条在125例正常人血清中均未出现,而第Ⅴ和第Ⅴ条在42例肝癌中有31例出现,但在70例非肝癌肝病病人中没有1例出现.因此第Ⅴ和第Ⅵ同工酶带可能是肝癌的特异带,会有助于肝癌的诊断。用同法又发现胎儿和新生儿脐带血清中存在着与肝癌病人血清中相对应的异常同工酶带,新生儿出生后这异常的第Ⅰ、Ⅴ和Ⅵ同工酶逐渐减弱,到10岁以后全部消失。这些结果提示肝癌病人血清中出现的5′-核苷酸磷酸二酯酶异常同工酶带属于癌胚同工酶。更多还原

【Abstract】 5′-nucleotide phosphodiesterase is-oenzymes have been separated from theserum of human hepatocellular carcinomaon 4-20% tubular polyacrylamide gelgradient electrophoresis showing ele-ven isoenzymes in which Band Ⅰ ,Ⅴ andⅥ (from anode to cathode) are allabsent in 125 normal sera.Band Ⅴ and Ⅵwere detectable in 31 (73.8% ) of 42patients with hepatocellular carcinomabut absent in 70 cases of other diseases.Therefore, Band Ⅴ and Ⅵ are possiblyspecific isoenzyme bands of primary liv-er cancer and might be a new additon tothe means in the diagnosis of this disea-se. By the same method, also found thatserum abnormal isoenzyme bands of pa-tients with liver cancer corresponded tothose of cord blood sera of the newbornand foetus. It has also been found thatthese abnormal isoenzymes are diminishi-ng gradually after birth and all disape-ar when children reach the age of ten.These findings indicate that the 5′-NPDaseabnormal isoenzymes belong to the carc-inoembryonic isoenzymes in the serum ofpatients with liver cancer.更多还原