【摘要】 A、B、E型肉毒素原品及透析液有色氨酸特征紫外和荧光光谱,透析品在紫外区无吸收和荧光,原品红外光谱有色氨酸特征谱带,透析品则无,证明透析品(毒蛋白)分子中不含色氨酸,原品中色氨酸是肉毒梭菌特有的代谢产物。A、B、E毒蛋白分子具有双链螺旋结构,双链间由—S—S—键连接,在85℃加热时,双链并不破坏。B的毒性与硫醚键和螺旋结构相关,A、E的毒性与分子肽链间结构变化有关。更多还原

【Abstract】 The initial samples of the A, B, E type botulins and their dia- lyzates have the characteristic UV and fluorescent spectra of tryptophan. The dialyzed samples have no absorption over ultraviolet area and do not emit fluorenscence. The tryptophan bands appear in the IR Specta of initial samples of A, B, E type botulins, and for the dialyzed samples do not. It is confirmed that the molecules of the dialyzed samples (toxicitic protein) of these botulins do not contain the tryptophan, and the letter in the initial samples is metabolites of clostridium botulins. The molecules of these botulins have bichain coil structure. The toxicity of the botulin B is dependent on the thioether bond and the coil structure, and that of A and E on the structural change of peptide Chain in their molecules.更多还原