【摘要】 本文对源于微生物的Ｓ－２４糜蛋白酶抑制剂抑制糜蛋白酶的动力学进行了研究。动力学研究表 明Ｓ－２４酶抑制剂抑制糜蛋白酶，对底物酪蛋白、血红蛋白的关系均为竟争性抑制。当Ｓ－２４酶抑制 剂浓度增大时，酶水解酪蛋白的反应速度降低了。反过来，如果抑制剂的浓度保持一定，那么抑 制剂的活力则随着底物浓度的增加而降低。当以酿蛋白和血红蛋白为底物时，抑制常数Ｋｉ值分别 为４．０×１０－９和２．４×１０－９。更多还原

【Abstract】 The Kinetics of chymotrypsin inhibition with microbe-produced S-24 en- zyme inhibitor is presented in this paper. Kinetic analysis revealed that the inhibition on chymotrypsin by S-24 enzyme inhibitor is competitive with res- pect to the substrate. When the concentration of S-24 enzyme inhibitor incr- eased, the reaction rate of hydrolysis of substrate by chymotrypsin decrease ed. Conversely under a defined concentration of the inhibitor, the activity of the inhibitor decreased as the substrate concentration increased. When casin and hemoglobin were used as substrate, the Ki value were 4.0× 10-9anp 2. 4× 10-9 respectively.更多还原