【摘要】 从东北地区分离的快生型大豆根瘤菌QB113(fast-growing R.japonicum QB113)在酵母——甘露醇液体培养基上生长时,世代时间为3.22小时,代谢过程产酸,生理生化特性与国内外报导的快生型大豆根瘤菌相类似。我们首次报导了快生型大豆根瘤菌对栽培大豆表现的良好共生固氮能力和增产效果。在实验室的无菌水培实验中,铁丰18大豆接种QB113,种子产量比不接菌的对照提高121.7％,比接种快生型大豆根瘤菌USDA193的提高55.5％,比接种慢生型大豆根瘤菌61A76的提高9.1％,在黑龙江省莞达山南麓(白浆土地区)、北麓(黑土地区)的田间小区试验和大面积(20公顷)试验中,对黑农26、合丰25、82—7799—2大豆进行同样的接菌处理,接种QB113的种子产量比对照分别提高8.6％、10.0％和15.7％。结果说明快生型大豆根瘤菌QB113是可以与栽培大豆品种形成有效共生的。并且,在生产应用中具有一定的价值和可观的前景。更多还原

【Abstract】 Fast-growing R. japonicum QB113 was isolated from nodules of wild soybean(Glycine soja) growing in northeast china. In yeast extract mannitol liquid medium its mean generation time was 3.22 hours and it produced acid. Its physiological-biochemical characteristics were similar to other fast-growing R. japonicum reported. For the first time it was reported that R. japonicum QB113 showed both good infectiveness and effectiveness with common commercial cultivars of soybean in the greenhouse and field experiments. In sterile liquid culture experiment, seed yield of Tiefeng 18 inoculated with QB113 increased 121.7% than that of control, and 55.5% than that of plants inoculated with fast-growing R. japonicum USDA193, and 9.1% than that of plant inoculated with slow-growing R. japonicum 61A76. The same treatments were conducted on Heinong 26, Hefeng 25, 82-7799-2 soybean varieties in field experimental plot, and 20 hectare in the South(planosol area) and North(black soil area)of Wan Da mountain, Heilongjiang Province. The seed yield of plant inoculated with QB113 increased 8.6%, 10.0% and 15.7% respectively than that of uninocu-laed. The results of our experiments showed that the fast-growing R. japonicum QB113 can form effective symbiosis with the soybean culti-var, and proposed to a definite valuable prospects in increasing the yield of soybean production.更多还原