【摘要】 本文报道了柞蚕肠道致病菌、灭活的柞蚕病毒和微粒子孢子以及柞蚕自身物质诱导柞蚕蛹产生抗菌物质的试验.并与大肠杆菌(Escherichia coli K-12D31)的诱导活性进行了比较;应用酸性聚丙烯酰胺凝胶电泳对上述诱导源诱导产生的抗菌物质进行了鉴定和比较,初步表明:1.柞蚕致病菌、灭活的柞蚕病毒和微粒子孢子以及柞蚕自身物质均能诱导柞蚕滞育蛹产生抗菌物质,但其活性差异显著,2.上述诱导源诱导柞蚕蛹产生抗菌物质不同组份的相对量和活性在体内持续的时间有明显差异,3.同一诱导源诱导柞蚕蛹产生抗菌物质的不同组份不是同时产生的.也不是同时消失的.更多还原

【Abstract】 This paper described that fivespecise of intestinal pathogenic bacteria of Chinese oak silkworm, including the pathogenic bacteriumof-Empty-Gut disease of A. Pernyi, Streptococcus pernyi, Could induced thediapausing pupae of A. Pernyi to produce antibacterial substances in the hemolymph. The antibacterial substances of A. Pernyi pupae induced by the intestinal pathogenic bacteria had been compared with that by Escherichia coli K-12 D31. It was found that the antibacterial activity induced by the intestinal pathogenic bacteria was much higher than that by E. coli. and the period of antibacterial activity lasted in the hemolymph of the pupae induced by the pathogenic bacteria was also much longer than that induced by E. coli. The polyacrylamide gel eletrophoresis showed that the antibacterial substances induced by injecting with two species of the intestinal pathogenic bacteria had higher ratios in P5, P9a and p9b, and it was also observed that the different components of antibacterial substances was not appearing and disappearing at the same time in the hemolymph of the pupae after it was induced.更多还原