【摘要】 正常小鼠肝脏能贮溜大量HpD,当用320～350J/cm~2剂量的激光照射时即发生光敏效应。肝细胞最早发生改变的是线粒体和糖原,继而内质网、溶酶体、细胞核及血窦面的细胞膜等也发生明显的变性坏死变化。血窦内的枯否细胞变化也很明显,内皮细胞也相继发生改变。从实验结果表明,光敏效应在细胞的膜结构、核染色质等都起反应,支持单态氧学说,并为临床应用提出了应注意的问题。更多还原

【Abstract】 The liver, a complex structural and functional organ was used to study the ma-chanism of HpD-laser photo-dynamic effect.seventy two hours after HpD 10 mg/kg was injected into the caudal vein of the mouse the liver areas were radiated by the dye-laser 20 minutes through the skin, (wave length of 630 nm,output power of 250-270mW, spot size of 1.ocm in diameter, dosage of 320-350J/cm2) .After radiation the liver tissue was observed under light and electron microscopes at 30 min and by the end of the ist, 2nd, 4th, 12th and 24th hours respectively with general technique.The earliest signs of injury in the cytoplasm of liver cell were the prompt di-sapperance of glycogen and then swelling of the miotchondria. After 4 hours the membrane-bounded vacuoles were formed and the granular endoplasmic reticulum changed progressively.In the kupffer cells the lysosomes changed rapidly and evidently.This experiment showed that the liver has restored a large amount of HpD in the cells. When the liver is radiated by Laser the photo-sensitive effects would lead to necrosis.In the earlier stage some disturbance of liver sinuses occurs and this influences the microcirculation and promotes injury to the liver cells.更多还原