【摘要】 石蜡切片α-乙酸萘酯酶(ANAE)染色法用于研究北京鸭胚及胚后早期腔上囊发育的不同阶段。观察了淋巴细胞的ANAE反应特点,并将滤泡上皮和网状上皮成分的ANAE反应与对应的碱性磷酸酶(ALP)染色对比,结果表明:T型ANAE阳性淋巴细胞最初出现在胚胎28天。孵出后,数量略有增加。淋巴滤泡的联滤泡上皮,联滤泡上皮下方以及髓质中央的网状上皮细胞呈M型ANAE阳性,滤泡髓质边缘层及近边缘层的网状上皮细胞ALP阳性,ANAE阴性。值得了注意的是在胚胎发育中,酶染特性不同的两种上皮细胞分别来自于粘膜上皮的表层和基层。讨论了B淋巴细胞在腔上囊中发育的微环境。更多还原

【Abstract】 Acid α-Naphthyl acetate esterase (ANAE) method was used to paraffin section to study the ANAE activity and distribution in the developing bursa of Fabricius of White Peking Duck in embryonic and duckling stages, The results indicate that 1. "Tlike" ANAE positive lymphocytes first appear in the follicles of 28-day embryos and its number increases slightly after hatching. 2. In the development of the lympho-epi-thelium follicle, the surface and basal layer of mucosal epithelium are tending to different directions of differentiation. Comparing the results with that of ALP, it is interesting to note that the follicle-associated epithelium (FAE) and the reticulo-epi-thelial cells in the center of the follicular medulla are "M-like" ANAE positive in certain stages, the basal layer of the mucosal epithlium, the marginal layer of the follicular medulla and the reticulo-epithelial cells near the marginal layer are ALP positive, but ANAE negative. The above results were discussed in relation to the mi-croenviroment for B lymphocyte development.更多还原