【摘要】 应用限制性内切酶图谱分析法,结合Southern印迹法和核酸杂交技术,对茶毛虫、棉蛉虫,油桐尺蠖、斜纹夜蛾以及蓖麻蚕等5种昆虫的7株核型多角体病毒DNA,进行了基因组同源性测定。结果表明,不同种昆虫多角体病毒DNA的酶切图谱不相同,DNA片段与不同源的DNA标记探针之间无杂交带出现。而同种昆虫病毒的不同分离株间,除少数DNA片段的电泳迁移率稍有不同,以及出现一些互不相同的亚克分子带之外,它们的DNA酶切图谱基本一致,並且几乎所有片段都可与同种的标记探钟杂交。对一些DNA片段迁移率的改变及亚克分子带出现的原因进行了讨论。更多还原

【Abstract】 The genetic homology of seven NPV genomes have been studied by restriction enzyme pattern analysis and DNA hybridization utilizing 32P-label-led NPV-DNA as probes. The resits showed that the restriction enzyme patterns of NPV-DNA among five NPV infecting different host species were different, but those between isolates from the same host species were almost the same except for some fragments with different migration rate and some submolar fragments. Detection of genetic homology by DNA hybridi-zation revealed that the major DNA fragments of NPV from the same host species hybridized with homologous probes. No hybridization can be observed between NPV-DNA from different host species. This report suggests that restriction enzyme analysis and molecular hybridization are of value for characterization of this group of viruses.更多还原