节点文献
新疆石河子地区印度麻花叶病植株病毒分离物的鉴定
IDENTIFICATION OF A VIRUS ISOLATE FROM SUNN CROTALARIA WITH MOSAIC DISEASE IN XINJIANG SHIHEZI
【摘要】 1984年,从新疆石河子农学院实验站印度麻花叶病植株上,分离到一株病毒分离物Sc-1,经汁液摩擦接种试验表明,它可以侵染10种豆科植物和2种藜科植物。在印度麻、蚕豆、豌豆、箭舌豌豆、扁豆、山藜豆、田菁和红三叶草上引起系统花叶,在豇豆上产生局部枯斑和系统花叶,在苋色藜、昆诺藜上表现为系统黄斑。失毒温度为55~60℃,稀释限点10-3~10-4,体外保毒期3~4天。可经汁液和蚜虫传播,不通过种子传毒。病毒粒体为线条状,大小为13~15×750nm。光学显微镜检查可见,病叶表皮细胞内形成不定形的内含体。电镜下可见风轮状、环状内含体。分离物Sc-1与菜豆黄色花叶病毒(BYMV)抗血清呈阳性反应。我们将Sc-1归为菜豆黄色花叶病毒(BYMV),且为豇豆株系。
【Abstract】 A virus isolate was obtained from sunn crotalaria ( Crotalaria juncea ) with mosaic symptom in Shihezi, Xinjiang. This isolate could infect 12 species of both Leguminosae and Chenopodiaceae, causing [systematic mosaic in Crotalaria juncea, Vicia faba, Pisum sativum, Vigna sesquipedalis, Trifolium pratense, and systematic yellow spotting in Chenopodium amaranticolor and Chenopodium quinoa.Iis thermal inactivation point was 55-60℃, dilution end point was 10-3-10-4 and longevity in leaf extract was 3-4 days. This virus was transmissible by sap inoculation in the laboratory and by aphids but not by seeds. The filamentous viral particles were 750nm in length and 13-15nm in diameter. Inclusion bodies without definite form in the epidermal cells of the infected leaf were observed under microscopy. Typical pinwheel inclusion bodies in the cytoplasm of the ultra-thin sectioned diseased leaves were very similar to those of the Potyvirus group. This virus could react with Bean Yellow Mosaic Virus ( BYMV ) antiserum. On the basis of these characteristics, it could be identified as Cowpea strain of BYMV.
- 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,1985年04期
- 【被引频次】2
- 【下载频次】31