【摘要】 用Maxam-Gilbert化学切断法,从含全长病毒DNA的克隆pCaMV C17中,测定了CaMV-XJ基因组的全部核苷酸序列。CaMV-XJ DNA含有8,060个碱基对。在不同的译读相位中,CaMV基因组含有6个主要ORF或可能的基因,与其他巳作全序列测定的三个CaMV分离物比较,有三个显著的差别;(1)ORFⅥ编码的氨基酸变化达16％,显著高于其他区域的变化和其他三个分离物之间的差别,(2)在ORFⅣ的近氨基末端有39bp的插入,它与被插入区的序列几乎是直接重复;(3)由于核苷酸置换而引入了终止信号,所以T.Hohn[2]推测,可能存在的ORFⅦ不可能编码有功能的蛋白。更多还原

【Abstract】 The complete nucleotide sequence of CaMV-XJ genomic DNA has been determined by Maxam-Gilbert chemical cleavage method from a clone pCaMV Cl7 which contains the full-length viral DNA copy.The entire sequence is 8060 base-pair long. There are six major open reading frames ( ORF)or putative genes in CaMV genome using different reading frames.In comparison with other three completely sequenced CaMV isolates, the following marked differences were observed;(1) the amino acid change in ORF Ⅵ was about 16%, considerably higher than that occured in other coding regions and that observed among the other three isolates; ( 2 ) an insertion of 39 bp at amino-proximal region of ORF Ⅳ, its Sequence was almost the same as that of the target region; ( 3 ) introduction of two in-phase termi-n ation codons in ORF Ⅷ suggested by T.Hohn which made it imposs be to code for a functional protein.更多还原