【摘要】 用CV-1细胞从急性腹泻病儿7份粪便标本中直接分离出4株人轮状病毒（HRV）,并适应传代14代。感染细胞出现特征性细胞病变,经电镜、ELISA、免疫荧光染色及病毒RNA电泳等试验证实HRV毒株在CV-1细胞中的繁殖及抗原特异性。病毒滴度为10^6.0TCID₆₀。分离的4株HRV毒株均为RNA电泳型长型,经CV-1细胞传7～14代后,RNA图型与原粪样相比未见变异。更多还原

【Abstract】 Four human rotavirus strains were isolated from 7 fecal specimens of pediatric patients with acute diarrhea and underwent 14 passages in CV-1 cell culture.The replication and specificity of these isolates in cell culture were confirmed by characteristic cytopathic effects, EM, ELISA, IF and viral RNA electrophoresis. All of the 4 isolates were long electrophero-type and no difference in RNA patterns was observed between the original fecal extract and the strain adapted in CV-1 cell culture for 7-14 passages.更多还原