【摘要】 本文报导仙灵脾和肉苁蓉对人淋巴细胞E花结形成和酸性α—醋酸萘酚酶酯酶（ANAE）活性的影响.正常人外周血淋巴细胞与药物在体外37℃共温1小时后测定活性E（Ea）和总E（Et）花结率,花结涂片作ANAE染色.结果表明,阳性对照药猪胸腺素F5（500μg/ml）、左旋咪唑（10μg/ml）和黄芪低浓度（5mg/ml）时,补肾药仙灵脾和肉苁蓉低浓度（5mg/ml）时均能增加Ea花结率,但对Et花结率无影响.而黄芪、仙灵脾和肉苁蓉高浓度（50mg/ml）时均可降低Et花结率.实验中未见猪胸腺素F₅和左旋咪唑能增高ANAE⁺淋巴细胞百分率,但黄芪、仙灵脾和肉苁蓉在高浓度或低浓度时可降低ANAE⁺淋巴细胞百分率.本文结果提示补肾药仙灵脾和肉苁蓉在一定浓度下能促进Ea花结形成,但抑制淋巴细胞ANAE活性的作用需进一步探讨.更多还原

【Abstract】 The human lymphocytes of peripheral blood from normal subjects were incubated in vitro with different drugs at 37℃ for 1 hour. The active E-rosctte ( Ea ) and total E-roscttc ( Et ) formation were performed, and the staining for ANAE smears were examined. The positive control drugs, i.e. pig thymosin F5 ( 500μg/ml ) , levomisolc(10μg/ml ) and Astragalus at low concerntration (5mg/ml ) increased the percentage of Ea+ cells but had no affect on Et+ cells. At low concentration ( 5mg/ml ) the Chinese drugs invigorating kidney such as EPimedium and Cistanche, showed the same effect as the positive drugs. At high concerntration ( 50mg/ml ) , however, Astragalus, EPimedium and Cistanche decreased the percentage of Et+ cells. Pig thymosin F5 and levomisole caused no further increase in the percentage of ANAE+ lymphocyte, whereas Astragelus, EPimedium and Cistanche decreased the percentage of ANAE+ lymphocyteat at both concentrations. These results suggest that EPimedium and Cistanche have potent capacity to promote Ea rosette formation of lymphocyte, but thei suppressive effects on ANAE activity of lymphocyte remain to be investigated.更多还原