【摘要】 EHFV在RLC中,不同毒株及感染剂量均能在感染后6～10天达繁殖高峰;33℃比37℃培养好;先在37℃后转33℃培养利于提高病毒产量;维持液中有否小牛血清对病毒繁殖无明显影响;冻融对提高病毒液度无好处;在RLC系统中EHFV 繁殖未达满意高滴度。更多还原

【Abstract】 Growth conditions of EHF virus in primary rat lung cell (RLC) culture were studied. The preliminary results were as follows : (l) different virus strains at various infective doses could multiply to relatively high tilers in 6-10 days after infection; (2) multiplication of EHF virus was much better at 33℃ than that at 37℃ and if EHF virus was first cultivated at 37℃, and then transferred to 33℃, better multiplication might be obtained, (3) the tilers of EHF virus in the maintainance medium of infected cell cultures were similar whether the maintainance medium contained calf serum or not, (4) treatment of the whole cell culture with repeated freezing and thawing failed to increase the virus yields. In primary RIC culture system, conditions used in these studies could not give the expected high titer desired for vacine produchtion and further studies for the optimal growth conditions are still needed.更多还原