【摘要】 本文报道 HBN-DNA 的提取纯化与克隆株的建立。此HBV-DNA 来自我国北方肝炎高发区带毒者血清,属 adr亚型,命名为 pHBV-NC1重组质粒;酶切、杂交与电镜观察均证明它含有 HBV-DNA 全基因组;在定出重组方向后,又从此重组质粒改建了除去部分 HBcAg 的HBsAg 亚克隆株,命名为pHBV-NCsAg。更多还原

【Abstract】 An adr sero-type HBV-DNA was isolated from serum of Dane’s particlea carrier in North China. The clone pHBV-NC was constructed by ligating vector pAT-153 and HBV-DNA after digestion with BamH1 and identified by restriction endonuclease mapping, hybridization and electron microscopy. After the orientation of HBV-DNA was determined, a subclone,pH BV-NC-sAg containing surface antigen gene only, was constructed by cutting off the main part of core antigen gene.更多还原