【摘要】 <正> 乙型肝炎病毒DNA是环状的,长度为3.2kbp。在克隆化并搞清乙型肝炎表面抗原(HBsAg)及核心抗原(HBcAg)的编码顺序后,许多实验室分别进行了表达的实验。HBcAg在大肠杆菌中的表达一直较顺利,但HBsAg则相反,即使用很强的启动子也很难得到满意的表达。HBsAg的分子量为24000左右。但更多还原

【Abstract】 The hepatitis B surface antigen （HBsAg） cDNA, containing the complete coding sequence, was ligated to the Lac promotor in a plasinid at the right orientation by ECoRI linker. The chimeric DNA was then used to transform E. Coli RRI. The expression of HBsAg was detected by ³⁵S-methionine incorporation. The result showed the expressed HBsAg has a molecular weight of 24000, and is very close to the molecular weight of the native HBsAg.更多还原