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分光光度法测定发酵液中卡那霉素A和B
SPECTROPHOTOMETRTC DETERMINATION OF KANAMYCIN A&B IN CULTURE BROTH
【摘要】 本文报道复杂组分中卡那霉素A和B的单组分的分光光度测定法。样品中的卡那霉素A和B用硅胶H薄层层析分离,以茚三酮-醋酸镉溶液系统显迹和显色。分离和测定过程很简单,容易操作。可供发酵液中卡那霉素A和B单组份的含量测定,并得到满意的结果.
【Abstract】 A simple, selective and accurate spect-rophotometric method for individual determination of Kanamycin A and B in culture broth is described. These components can be separated by thin layer chromatographyon silica gel H plates. Ninhydria-cadmium acetate reagent system is used for monitoring the color-producing reaction. The separation and detection procedure are easy to perform.
- 【文献出处】 抗生素 , 编辑部邮箱 ,1984年04期
- 【被引频次】1
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