【摘要】 1983年7～9月间,对我国滇南地区5例自然感染的间日疟病例,以驯化的中华按蚊为媒介,每8小时离体吸血1次,以卵囊阳性率和感染度(每阳性蚊卵囊均数)判定配子体活力的强弱。结果发现间日疟原虫配子体活力有明显的周期性,蚊媒的感染高峰似见于病人血液中出现发育中滋养体和成熟滋养体为主的发作间歇期,低谷则见于以成熟裂殖体和幼年滋养体为主的临床发作期,显示配子体的活力周期与无性体的裂体增殖周期有相当密切的关系。除1例外,在实验过程中均见一完全不能使蚊媒感染的无感染期,由此证明,周围血液内的配子体对蚊媒并不始终均具感染性。从蚊媒感染率的消长曲线和各期无性体组成的相互关系推断,配子体活力周期比无性体的裂体增殖周期略长。更多还原

【Abstract】 Anopheles sinensis were fed through membrane on blood from five patients naturally infected with vivax malaria in the southern pfert of Yunnan Province. After feeding at 8-hour intervals for 48 hours., all unfed mo’squitoes were removed from cages and the engorged ones were incubated at 26±1℃ and 70-80% RH on 10% glucose. 13 to 100 mosquitoes from each batch were dissected according to the level of infection 6-7 days later and the number of oocysts were recorded. The percentage of mosquitoes infected and the mean number of oocysts per infected gut were taken as critaria of the viability of the gametocytes. Both thick and thin, blood films were taken at the time when mosquitoes fed, and the number of asexual parasites and gametocytes were counted by the routine method.The general conclusions drawn from our work are: (1) The viability period of the gametocytes was demonstrated, but the nocturnal periodicity of morphologically mature gametocytes, as previously noted by Hawking et al., was not regularly observed. It is doubtful whether Hawking’s work on monkey infections could be applied to human Plasmodium vivax. (2) There is no marked and definite correlation between the infecti-vity of mosquitoes and the total parasite count or gametocyte density. (3) The infectivity of gametocytes for mosquitoes reached a peak at the interschizogony period and fell to low level at the time when schizogony occurred. (4) The time required for the rise and decline of gametocyte viability is little longer than the time required for an schizogony cycle.更多还原