【摘要】 本文详细地介绍了以鸡血为原料用超滤器制备鸡转移因子(TFu)的方法,并对转移因子的生物学特性、理 化性质,以及应用酸性α-醋酸萘脂酶(Acid a-naphthyl ecetate estenase,简称ANAE>标记T淋巴细胞测 定细胞免疫功能。此外,还使用TFu来预防人工复制的禽巴氏杆菌病及治疗鸡白痢病试验。结果表明,TFu蛋白 定性为阴性,本品中含有多肽、RNA、DNA以及赖氨酸、丙氨酸、脯氨酸等十七种氨基酸.每毫升注射液含多 肽786±0.75μg,RNA201.22±19.7μg,DNA340±12.2μg,动物试验安全,无抗原性及过敏性.临床应用后, 明显地提高了外周血中T淋巴细胞的百分率,增强和调整了机体免疫功能,对预防禽出败人工复制病例和治疗鸡 白痢病,有明显的效果.更多还原

【Abstract】 Transfer factor ( TF ) is a dialysable ( ultrafilterable ) extract of sensitised leukocytes. It transfers cellular immunity from a skin test positive donor to a skin test negative recipient. Its molecular weight is less than 5000 and consists of polypeptides RNA and DNA. In this paper the ultra-filtration for preparation of Transfer Factor from the normal fowl blood was described in detail. Some physicochemical and biological properties of TFu ( which were prepared by the method mentioned above ) were studied, and also TFu was used for prevention of fowl cholera and treatment of pullorum disease. It was shown that the TFu preparation was non-toxic, non-anaphylactic, non-antigenic, and protein-free. It contained polypeptides, RNA, DNA and 17 kinds of amino acids such as lys. ALA. PRO and so on. Each millilitre of the preparation has 786±0.75ug of polypeptioles 201.22 ± 19.7ug of RNA and 340 ± 12.2ug of DNA. In clinical use, the monber of lymphocytes in the peripheral blood was increased. The effect of TFu for preventing fowl cholera and treating preventive and therapeutic effects in Cholera Avium Pullorum disease seemed to be distinct.更多还原