【摘要】 本细胞株SGC—7901是从一例胃癌患者外科手术切除标本中获得的转移淋巴结,在离体条件下应用静置培养而建成的。培养液用RPMI—1640内含40％小牛血清,并加适量抗菌素,胰岛素和萄葡糖。所用小牛血清选用少溶血或无溶血者配制培养液,Hank氏液及其他试剂所用水均为三蒸馏水。培液pH维持在6.8～7.0范围内。细胞形态为上皮性。在传代后4天时,正常和异常有丝分裂指数高达55‰,中期分裂相多于其他各期。染色体主流数为不足三倍体值。细胞的异种移植长瘤率高。接种4只小鼠都见肿瘤生长,细胞是多边形,大小不一,部分细胞内纤维组织包围成巢可见到典型的腺癌结构。上述本株细胞特征与手术切除标本十分相似,具有胃腺癌特征,本株细胞肯定为胃癌细胞。更多还原

【Abstract】 A SGC-7901 cell line was successfully established from gastric carcinoma specimen of a female patient. The culture medium used was RPMI-1640 which contained 40％ of less-hemolytic or non-hemolytic fetal calf serum with sufficient amounts of antibiotics, insulin and glucose prepared with aqua tridistillata in Hank’s solution and other re-agents, and maintained at pH 6.8-7.0.The cells were epithelial in nature. On the fourth day of transfer, the mitotic (normal and abnormal) index showed a rapid increase to as high as 55%. The percentage of metaphase was higher than that of other phases. The chief configuration of the chromosomes was superdiploid. Growth rate of the tumor in various animal species was high. The growth of carcinoma was observed in all of the four rats inoculated, and that the cells were polygonal in shape of varying sizes. A. number of cells were enclosed in fibrous tissue forming nests with typical structure of adenocarcinoma. The above mentioned characteristics of SGC-7901 cells showed a striking similarity to those of the original surgical specimen.更多还原