【摘要】 作者用气相色谱法测定了人体血中α-生育酚的浓度。经过预处理去除血清中对α-生育酚测定有干扰的胆固醇后进行色谱分析。α-生育酚醇化为α-醋酸生育酚后保留时间约为8分钟,内标准采用三十四烷。本法回收率平均为76.4％,变异系数(CV)=4.4％(n=10)。25例脐血中α-生育酚测定值平均为2.1±0.5μg/ml,与分光光度法测定结果显著相关(r=0.6803,p<0.001)。更多还原

【Abstract】 The estimation of a-tocopherol in human blood was carried out by gas chromatography. The cholesterol in plasma must he removed in advance. The a-tocopherol is chromatographed as tocopherol acetate and is eluted with a retention time of about 8 min. Tetratriacontane was used as internal standard. The average recovery rate was 76.4 per cent and coefficient of variation was 4.4 per cent (N = 10). The mean level in 25 cord blood was 2.1 ×0.5 μg/ml. It correlated well with the results by spectrophotome-tric method (r=0.6803 P<0.001).更多还原