【摘要】 <正> 酞丁安(3-phthalimido-2-oxo-butyraldchyde-bis-thiosemicarbazone,V6133)是我们自己设计合成的一种缩氨基硫脲类化合物。实验室及临床研究证明有抗沙眼衣原体活性的作用,对其生产工艺和临床疗效已进行了鉴定。最近临床试用认为,对带状疱疹及单纯疱疹有一定的疗效,但目前尚未见实验室的研究报道。为了探索酞丁安的抗病毒活性,我们用组织培养细胞研究了对HSV-1及HSV-2复制的抑制作用,及其对正常细胞的毒性。结果报道如下。更多还原

【Abstract】 PR assay, YR assay and CPE inhibition assay were used to determine the effect of Tai-Ding-An (3-phthalimido-2-oxo-butyraldehyde-bis-thiosemicarbazone, V 6133) against HSV-1 and HSV-2 in vitro. The results of PR assay showed that the ratio of the plaque number was near to zero in the presence of drug concentrations of more than 50μg/ml in comparison with DMSO control. Dose-response curve obtained by YR assay revealed that Tai-Ding-An markedly inhibited replication of both HSV-1 and HSV-2. PRK cells were treated with 100 μg/ml of Tai-Ding-An and incubated for 2 days, no HSV-induced CPE was observed while CPE was very evident in the control. No CPE was observed for up to 5 days when the PRK cells were treated continuously with the media containing 100 μg/ml of the drug and changed daily. From these results it would appear that Tai-Ding-An is an effective agent against HSV-1 and HSV-2.In vitro experiments also showed that the toxic effect was mild when the concentration of Tai-Ding-An was less than 100 μg/ml.更多还原