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银杏组织培养的初步研究

A PRELIMINARY RESERCH ON TISSUE CULTURE OF GINKGO BILOBA LINN.

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【作者】 罗紫娟;

【Author】 Luo Zijuan (Dept, of Biology, Guangxi Teacher’s College)

【机构】 广西师范学院生物系;

【摘要】 用银杏(Ginkgo biloba Linn.)种子胚剪成2—3段,培养在White+2,4-D5毫克/升+NH4Cl5.34—53.49毫克/升+LH200—400毫克/升的培养基上,诱导愈伤组织形成,并在培养10—25天后在胚轴的伤口上形成芽,再转移到加有0.05%活性碳的上述培养基上,6—10天后生根长成完整植株。

【Abstract】 The seed plumule of Ginkgo biloba Linn.was cut into 2-3 sections and cultivated in the culture medium White+ 2, 4-D2 mg/litre + NH4Cl 5.34-53.49 mg/litre + LH200-400mg/litre and then induced the formation of callus, the buds on the pumular axis of each section were formed in 10-25 days, and then they were transplanted to the above-mentioned medium supplemented with 0.05% of activted carbon. After 6-10 days these buds will take roots and grow up to form plants.

  • 【文献出处】 云南植物研究 ,Acta Botanica Yunnanica , 编辑部邮箱 ,1984年01期
  • 【被引频次】60
  • 【下载频次】232
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