【摘要】 本文以中国仓鼠肺细胞Wg3-h(HGPRT~-)和正常人的淋巴细胞作为亲本,借助仙台病毒促融,HAT培养液选择得到FD1杂种细胞。通过观察细胞形态、Giemsa-11分化染色,G-分带、G-6-PD和LDH同工酶的电泳等一系列鉴定指标,以及细胞周期的研究,证实FD1细胞是包含全套中国仓鼠染色体组和少数几条人的染色体(5、11、12、21、22、xq~-、Y等)的杂种,其中还存在中国仓鼠和人体染色体的易位。FD1等杂种细胞的建立将有助于进行人类基因制图以及人体基因的结构功能和表达调控的研究。更多还原

【Abstract】 Chinese hamster cell line Wg3-h (HGPBT-) and normal human lymphocytes were used as parental cells for the fusion experiment in the presence of UV-inactivated Sen-dai virus. The resulting FD1 hybrid after being selected in HAT medium containing hypoxanthine, aminopterin and thymidine retained a complete Chinese hamster genome plus a few human chromosomes (No: 5, 11, 12, 21, 22, Y, Xq- ...) . Besides, transloca-tions between Wg3-h and human chromosomes were observed. Cell morphology, Gie-msa-11 staining, G-banding, glucose-6-phosphate dehydrogenase (G-6-PD) and lactate dehydrogenase (LDH) isozyme eletrophoretic analyses were used as criteria in identifying the hybrid.These results obtained provide a valuable information for obtaining and identifying rodent-human somatic cell hybrids and will contribute to the mapping of human genes and the understanding of the structure and function of human genome.更多还原