【摘要】 本文以质粒pAT153为载体,以E.coli SK1592为受体菌,将北京鸭肝脏mtDNA的2.17kb的EcoRI/BamHI限制片段进行了克隆,并对重组质粒作了限制性内切酶分析、Southern吸印与杂交分析。此外,还对重组体的一些性质作了初步研究,并对鸭肝mtDNA的另一个EeoRI/BamHI片段(2.81kb)不能被克隆的原因进行了讨论。更多还原

【Abstract】 The 2.17 kb EcoRI/BamHI restriction fragment of the mtDNA from Beijing duck liver was cloned, using plasmid pAT153 as a vector and E. coli SK1592 as recipient cells. The recombinants have been identified by restriction analysis and Southern blot analysis. Moreover, the stability of the chimeric plasmids in recipient cells has been studied and the question of nonsuccessful cloning of another EcoRI/BamHI fragment (2.81 kb) of duck liver mtDNA is also discussed.更多还原