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γ辐射引起花生和水稻种胚非按时DNA合成效应的研究

The Induction of Unscheduled DNA Synthesis in Embryos of Peanut and Rice by r-radiation

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【作者】 丘泉发; 刘振声; 李宝健; 罗进贤; 李爱群; 颜浩; 陈伟荣;

【Author】 Qiu Quanfa Liu Zhensheng Li Baojian Luo JinxianLi Aiqun Yan Hao Chen Weirong (Department of Biology, Chungshan University, Guangzhou)

【机构】 中山大学生物学系; 中山大学生物学系 广州; 广州; 广州; 雷州师范; 海南大学; 华南农学院;

【摘要】 用3H-胸腺嘧啶核苷渗入法,研究受60Co-γ射线各种剂量(10、20、30、40千伦,剂量率836伦/分)照射的花生和水稻种胚萌发早期DNA合成率的变动。结果表明,受γ辐射的花生和水稻种胚出现非按时的DNA合成,用咖啡碱能够抑制这种合成,这种非按时的DNA合成与γ辐射剂量有一定的关系。本研究工作证明了:在上述两种植物的细胞中具有修复DNA损伤的功能。

【Abstract】 The unscheduled DNA synthesis in embryos of peanut and rice irradiated with γ-ray was studied. The results showed that the rate of DNA synthesis during the early stage of soaking of γ-irradiated seeds (10-20 KB for peanut and 10-30 KB for rice) was larger than that of non-irradiated seeds and radiation with higher doses (30-40 KR) suppressed 3H-TdB incorporation. This kind of incorporation was inhibited by caffeine. This indicated that the γ-radiation induced incorporation of 3H-TdR during the early stage of germinating seeds might be correlated with DNA repair phenomenon.

  • 【文献出处】 遗传学报 ,Acta Genetica Sinica , 编辑部邮箱 ,1984年05期
  • 【被引频次】18
  • 【下载频次】21
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