【摘要】 以福建省牛流行性腹泻病牛的25份(水牛13份、黄牛12份)粪便标本中,用电镜和免疫电镜法检出具有典型牛轮状病毒颗粒的有8份(水牛5份、黄牛3份)粪便标本,在免疫电镜中观察到抗原和抗体形成“架桥”现象。24头牛人工饲喂病牛的无菌粪便过滤液,所发生的临床症状与自然病例是一致的。从试验腹泻的粪便中用电镜法又检出牛轮状病毒颗粒,同时在血清学上也证明是试验牛发病的病原。结果说明1980年福建省发生的牛流行性腹泻的病原是轮状病毒。更多还原

【Abstract】 Typical rotavirus paritcles were found by electron microscopy in 8 out of 25 including buffaloes fecal samples from diarrhoeic cattle. The"bridge"phenomenon had been observed with immuno electron microscopy. In experimental infection in 24 heads of cattle there is a profuse watery diarrhoea after oral inoculation of germ-free filtrate of the feces. The rotavirus particles were also found by electron microscopy from their feces and there were positive responses on serological tests.The results show rotavirus is the primary pathogen of cattle-epizootic-diarrhoea.更多还原