【摘要】 本文报道了提纯的μ₂ RNA在下述三种方法中的转染效率:（1）Ca²⁺处理,（2）高渗培养基,（3）硫酸鱼精蛋白存在下的溶菌酶-EDTA系统。实验结果表明采用第三种方法的转染效率最高,可达到2.1×10⁶ p.f.u/μg RNA。研究了硫酸鱼精蛋白在转染中的作用,并观察了RNA浓度,RNA末端部分降解,溴化乙锭（EB）,放线菌素D和热变性等对μ₂ RNA转染效率的影响。更多还原

【Abstract】 Three methods: 1) Ca²⁺ treatment, 2)hypertonic medium, and 3) lysozyme-EDTAsystem in the presence of protamine sulfatewere applied to compare the efficiency oftransfection of purified μ₂ RNA. Amongthem, the third method is the most effec-tive for transfection with plaque formingunits （p. f. u） reaching to 2.1×10⁶ per μgRNA. The action of protamine sulfate andother influencing factors on the transfectionof μ₂ RNA, such as the concentration ofRNA, the partial digestion of the RNA mo-lecule in 5’ or 3’ terminal ends, the integra-tion of ethdium bromide and actinomycinD, and the heat denaturation were also ob-served and discussed in detail.更多还原