【摘要】 对三组经培养和提纯的不同培养阶段的小鼠腹腔巨噬细胞,用形态学和细胞化学方法,通过细胞内酶活性变化来观察SiO₂对细胞的毒性效应。结果:①SiO₂组细胞吞噬SiO₂粉尘后存活时间较正常组缩短4～5倍。②SiO₂组细胞内酶活性较正常组显著减退,二组间差异显著。③在酶活性动态变化组中实验组细胞内酶活性较正常组为低,二组间差异也极显著。结果表明,细胞内酶活性减退与SiO₂对细胞的毒性效应有关。更多还原

【Abstract】 In order to study the pathogenesis of sllicosis,the cytotoxic effects of silica on the activity of the acid phosphatase(ACP)and nonspecific esterase(NSE)at various cultural stages of cultured and purified peritoneal macrophages of mice were observed with morphologic and cytochemical methods. The results showed that peritoneal macrophages in the silica group phagocytosed silica particles rapidly died and broke earlier than that in the normal group. The activities of the intracellular enzymes in the silica group were much lower than those of normal one. The differences of the activities of the relative enzymes between the normal and silica group cells were very significant (P<0.001).Furthermore, in the dynamic process of culture the activities of intracellular ACP and NSE of the cells which had phagocytosed silica were lower than those of normal ones in the same animal. The differences were also very significant ( P< 0.005). The above facts indicated that the decrease of activities of the intracellular ACP and NSE was related to the cytotoxic effects of silica panicles on macrophages.更多还原