【摘要】 用昆明种小鼠按Wasley’s法快速获取腹腔巨噬细胞。以贴壁法提纯(9～12℃),体外培养的细胞呈单层生长,可存活2～3周。对提纯的细胞用形态学、细胞化学、吞噬及抗胰蛋白酶消化试验等法鉴定。结果:体外培养的细胞具有巨噬细胞的典型特征,属高纯度的巨噬细胞,纯度达97%以上。使用高纯度的细胞必将提高实验研究的质量。更多还原

【Abstract】 In order to study silicosis, the enrichments of culture of peritoneal macrophages were observed in vitro. A number of peritoneal macrophages from mice were collected using Wasley’s method. Cell cultures were incubated in 199 media at thirty-seven degree centigrade for 2-3 weeks which grew up to be a monolyer of round or stellar cells with survival. By a series of morphological, cytochemical methods, phagocytosis and pepsin resistance tests had been used for identification. The results have shown that the cultivated macrophages maintain their typic characteristics in vitro. In this work the procedure led to a greater than 97% macrophages.更多还原