【摘要】 本实验用PEG介导,将小鼠BW₅₁₄₇细胞与小鼠网织红细胞在体外进行悬浮融合,经HAT选择性培养基筛选获得的胞质杂交细胞,表现出增殖减慢、生长率下降、生瘤能力降低等去恶化现象。本实验从cAMP-PDE细胞化学反应显示出胞质杂交细胞内cAMP-PDE活性比亲本BW₅₁₄₇瘤细胞有所减弱,而cAMP水平则有所升高,提示胞质杂交细胞恶性程度下降可能与细胞内cAMP-PDE活性降低和cAMP水平升高具有一定的关系。本实验的结果将为进一步探索促使肿瘤细胞逆转的可能途径提供一定的依据。更多还原

【Abstract】 The BW₅₁₄₇ Thymolymphosar-coma cells and Reticulocytes of mousewere fused by induction of polyethyleneglycol（PEG）.The cybrids were ob-tained by using HAT seletive medium. Apparent correlation betweencAMP level,cAMP-PDE activity andmalignancy in BW₅₁₄₇ cells,reticu-locytes and the cybrids fused betweenthem was observed. The results of this experimentshow that the cell mitotic index of theBW/R cybrids（0.008）are lower thanthat of the BW₅₁₄₇ cells（0.063）.Thetumorigenicity of the cybrids are lowerthan that of the parental tumor cells. At the same time the cAMP-PDEactivity of BW/R cybrids are weakerthan that of the BW₅₁₄₇ cells whichshow strongly positive by the cytochemi-cal studies both in light and electronmicroscopy.While the intracellularlevel of cAMP in the BW/R cybrids（34.52±2.73 P.moles/10⁶ cells）ishigher than that of the parental tumorcells which is 21.45±2.03 P.moles/10⁶cells by radioimmunoassay. These results suggest that theremight be some correlation between thesuppression of the malignancy of thecybrids,the decrease of cAMP-PDEactivity and with high level of cAMPin these cells.更多还原