【摘要】 本实验用管蛋白抗体间接免疫荧光细胞化学方法,观察了我国建株的人胃低分化粘液腺癌MGc 80-3,人胃腺癌SGC-7901,人鼻咽癌上皮样细胞CNE,人食管癌上皮细胞ECa-109,人肺鳞癌LTEP-78,人啼腺癌LTEP-a₁,人肺小细胞癌LTEP-p七株癌细胞和HeLa细胞,小鼠S₁₈₀-V肉瘤细胞的微管形态。与人的正常包皮成纤维细胞和食管上皮细胞内精细的CMTC结构对比,肿瘤细胞间期的胞质微管普遍有减少或缺如的现象。参考Brin-kley对微管免疫荧光染色图形的分型方法,我们将观察的各种微管染色图形归纳为四种类型,比较各种细胞群体内微管类型的分布。肿瘤细胞群体内多数为微管缺如型和稀疏型,未见典型的丰满型,而正常细胞群体内都是丰满型。同时,肿瘤细胞的MTOC区面积明显增大。分裂期的肿瘤细胞内,有丝分裂器纺锤体微管荧光形态与正常细胞的没有差别。本文对肿瘤细胞间期胞质微管减少和缺如以及MTOC区明显增大的现象及其可能的意义进行了讨论,认为这是癌变机制研究中值得深入探讨的重要课题之一。更多还原

【Abstract】 We previously reported that theintracellular microtubules of normalfibroblasts from foreskin of Chinesechild show specific staining patterns.We here demonstrate several fluores-cent staining patterns of cytoplasmicmicrotubule complex(CMTC)in wellcharacterized tumor cell lines includinghuman stomach cancer cell lines(MGc-80-3 SGC-7901),human esophagealcarcinoma cells(ECa-109),human na-sol pharyngeal carcinoma cells(GNE),human lung cancer cell lines(LTEP-78,LTEP-ai,LTEP-p),HeLa cells,andmouse sarcoma cells(S180-V).Fourmain patterns of GMTC phenotypestermed as FG-,DC-,SO-,and SR-type by Brinkley et al previously innormal and transformed cells are identi-fied in our experiment.The distri-bution of the CMTC phenotypes inpopulations of tumor cells is very dif-ferent from that of normal cells;thefull microtubule complex pattern(FG-type)represents the main CMTC pat-tern of normal cells while the dimini-shed microtubule complex pattern(DC-type)represents that of tumor cells.The results presented here suggest thatthere is a great difference in interphaseCMTC phenotypes between normaland tumor cells.A marked diminutionof microtubular tubulin fluorescencewith special MTOC appearance isshown in tumor cells.Since the ap-pearance of mitotic spindle microtubulesis similar in normal and tumor cellsaccording to our observation,the di-sturbance of interphase CMTC orga-nization is assumed to be the mostimportant problem in the studies ofmalignant transformation.Detailknowledge about the structure andfunction of MTOCs of malignantcells is of interest too.更多还原