【摘要】 本文对尿素在Folin-Ciocalteu法测定蛋白质浓度时的影响进行了定量的研究.加入尿素后会使被测蛋白质溶液与Folin试剂反应后的吸光度值增加.实验显示,尿素在蛋白质溶液中的含量即使低于0.5%时,也对显色后的吸光度值有影响.添加尿素和未添加尿素的标准牛血清白蛋白溶液的含量与吸光度的双对数座标作图所获得的两条直线,几乎是平行的.这说明尿素对被测蛋白质溶液的影响,从尿素浓度0.18%到2.88%是近乎不变的.更多还原

【Abstract】 A quantitative study of the effect of urea on Folin-Ciocalteu’s method indetermining protein solution has been carried out. When urea was added the opticaldensity would increase after the reaction of the protein solution under test andthe Folin reagent. According to the result of this experiment, the increase ofoptical density was found, even if the urea concentration was lower than 0.5%.Two nearly parallel lines for BSA solution with and without urea wereobtained in plotting the data by way of log protein concentration against log opticaldensity. This shows that the effect of urea in different concentrations (rangingfrom 0.18% to 2.88%) is nearly constant.更多还原