【摘要】 按照 Rockhill 等用金葡菌协同凝集试验(SPA-CoA)方法检测17例伤寒患儿(309份尿标本)尿内可溶性抗原,并与对流免疫电泳(CIE)和尿培养作比较。17例检测结果,三者阳性率各为94.1%、52.9%和64.7%;309份尿标本阳性率各为42.7%、11.0%和12.9%。6例肾病综合征患儿和50名健康对照组均阴性。用 SPA-CoA 检测,于病程第二周有部分患儿阳性,至病程第八周仍可有少数阳性者。提示此法具有特异性和敏感性,可用于伤寒的诊断。更多还原

【Abstract】 Detection of specific typhoid antigen in urine of patients by SPA-CoA technique (Rockhill) in 17 clinically and Widal reaction proved typhoid children were studied. Urine was collected daily during hospitalization and subjected to assessment of antigen by SPA-CoA and counter-current immuno-electrophoresis (CIE) as well as bacteriological culture.Six cases of nephrotic syndrome and 50 healthy subjects served as controls.Of the 17 typhoid children,SPA-CoA test was positive in 94.1%,CIE positive in 52.9% and culture positive in 64.7%.Of a total of 309 urine specimens,the positive rates were 42.7%,11.0% and 12.4% respectively while the controls were all negative. Hence SPA-CoA is superior to the other two methods.In general,SPA-CoA was positive during admission (2nd wk of illness) and was still positive at discharge (in the 8th wk).SPA-CoA test is thus a very sensitive and highly specific aid in the diagnosis of typhoid infection.更多还原