【摘要】 作者设计了测定家兔血浆中间霉素浓度的荧光光谱法,并测定了静脉一次给药后家兔体内阿霉素的药代动力学参数。分析步骤:在含不同量阿霉素的家兔血浆中加入饱和NaHCO₃溶液及正丁醇,振摇、离心后取上清液测荧光强度（λ_ex484nm,λ_em591nm）。实验结果表明:阿霉素投入量（4～10³ng）与测得的荧光强度间线性关系良好,阿霉素在家兔体内过程符合药代动力学开放型两室模型,血药浓度-时间曲线方程为 C（ng/ml）=2.9840e^{-0.1792t（min）}+0.04347e^{-0.001798t（min）}本法灵敏度高,线性范围大,专属性好,操作简便,可提供临床作阿霉素血药浓度监测之用。更多还原

【Abstract】 The plasma level of adriamycin (doxorubicin), an effective antioancer drug, was determined spectrofluorometrically. (Escitation and emission peak wavelengths were at 484 nm and 591 nm respectively.)The experimental data exhibited a good linear relationship between the logarithms of the fluorescent intensity and the amount of adriamyoin added to the blank plasma in the scale of 4ng-1000ng, r = 0.9989. The concentrations of adriamycin in blood samples obtained from 6 rabbits, to which adriamycin had been administered intravenously by rapid injection at a dose of 2 mg/kg, were determined. According to the time course of mean plasma concentration data, the pharmacokinetic characteristic of adriamycin was found to be two-compartment open model with the followingparameters: t1/2(α) =0.067h, t1/2(β) = 6.42h, k10 =0.293h-1,k12=5.857h-1,k21=0.256h-1,v0 = 660.8 ml/kg.更多还原