【摘要】 1.用Oligo（dT）纤维素亲和层析将新疆大麦条纹花叶病毒（BSMV-XJ）RNA分为poly（A）⁺（与Oligo（dT）纤维素结合的）RNA和poly（A）^-（不与Oligo（dT）纤维素结合的）RNA,其相对含量poly（A）⁺RNA占75%,poly（A）^-RNA为25%。2.用³²P标记法测定了BSMV（XJ）RNA中3′端poly A的链长分布,结果poly（A）⁺RNA带有数个至三十几个腺苷酸残基,而poly（A）^-RNA也含十个以下的腺苷酸残基。3.用系统感病寄主大麦进行侵染性试验表明,poly（A）⁺RNA与poly（A）^-RNA对大麦具有同等水平的侵染性。4.文中讨论了Oligo（dT）纤维素的分离效果以及所用测定poly（A）链长分布方法的可靠性。更多还原

【Abstract】 By means of oligo(dT)-cellulose affinity chromatography the intact RNA ofBSMV(Xinjiang strain) can be separated into unbound(poly(A)-RNA) and bound(poly(A)~+ RNA) fractions.The ratio of poly(A)~- RNA and poly(A)~+ RNA in thetotal BSMV-RNA is 1:3.We observed that the length distribution of poly(A) at the3′-terminus of poly(A)~+ RNA is from several to more than 30 residues of adenosineand that the poly(A)~- RNA has also a poly(A) tail of less than 10 residues ofadenosine.The term poly(A)~- RNA as used here therefore refers to RNA that doesnot contain sufficient poly(A) to bind to the affinity cellulose.The infectivity of poly(A)~- RNA from BSMV(Xinjiang strain) is similar to thatof poly(A)~+ RNA suggesting that the length 3′-terminal poly(A) is not essential forinfectivity of BSMV-RNA.:~更多还原